rat anti-b220 (Thermo Fisher)
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rat anti-b220
Rat Anti B220, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-b220/b220+antibody/pmc12223566-62-49-53
Average 90 stars, based on 1 article reviews
Rat Anti B220, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-b220/b220+antibody/pmc12223566-62-49-53
Average 90 stars, based on 1 article reviews
rat anti-b220 - by Bioz Stars,
2026-10
90/100 stars
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Staining:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Enzyme-linked Immunosorbent Assay:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Isolation:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Injection:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Expressing:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Fluorescence:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Irradiation:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Concentration Assay:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Protein Concentration:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Immunofluorescence:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Real-time Polymerase Chain Reaction:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Blocking Assay:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Incubation:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Infection:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Flow Cytometry:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Recombinant:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Labeling:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: MANN-WHITNEY:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: Two Tailed Test:Article Title: Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis. Article Snippet: The following antibodies and dilutions were used: chicken anti-GFAP (abcam, Cambridge, UK (Cat. No. ab4674); 1:500), rabbit anti-IBA1 (FUJIFILM Wako, Neuss, Germany (Cat. No. 019-19741); 1:500), rabbit anti-βIII-tubulin (abcam (Cat. No. ab18207); 1:2000), rabbit anti-CD3 (abcam (Cat. No. ab16669); 1:500), Article Title: CCR6 Deficiency Impairs IgA Production and Dysregulates Antimicrobial Peptide Production, Altering the Intestinal Flora Article Snippet: Sections were deparaffined, rehydrated, blocked with PBS containing 1% BSA, 5% normal donkey serum, and 0.2% triton X-100, and then stained with the following antibodies: goat anti-CCL20 (R&D systems), Article Title: Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice. Article Snippet: opyright a 2013 American Society for Inve ublished by Elsevier Inc. All rights reserved ttp://dx.doi.org/10.1016/j.ajpath.2013.04.006 Pharmacologic inhibitors of IkB kinase (IKK), especially IKK-b, have been developed to treat inflammatory diseases.. However, their interactions with components of the NF-kB pathways are not fully known in allergic diseases.. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanisms in the NF-kB activation system were active, we examined the role played by IKK components on mast cell degranulation using a murine ocular immediate hypersensitivity reaction model. Pharmacologic inhibition of IKK in mice caused paradoxical aggravation of the mast cellemediated immediate hypersensitivity reaction and up-regulation in the expression of inflammatory cytokines. Article Title: Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag -dependent immunodeficiency Article Snippet: The following primary antibodies were used: Article Title: Aggravation of conjunctival early-phase reaction by Staphylococcus enterotoxin B via augmentation of IgE production. Article Snippet: Purpose: To investigate whether Staphylococcus enterotoxin B (SEB) affects the early-phase reaction (EPR) in experimental conjunctivitis.. Methods: Nc/Nga mice were sensitized to ragweed (RW) or phosphate-buffered saline (PBS) in alum.. The mice were subsequently treated three times a day with eye drops adulterated with SEB or vehicle on postimmunization days 29 to 31. Article Title: Interleukin-6-induced neuroinflammation is exacerbated by subclinical levels of interferon-α Article Snippet: Sections were incubated in 0.3% H 2 O 2 for 10 min and blocked in 1% goat serum with 0.1% Triton X-100 and 0.05% Tween-20 in PBS for 30 min, then incubated with primary antibodies rabbit anti-Iba1 (1:500, 019-19741, Wako Pure Chemical Industries), rabbit anti-CD3 (1:200, ab16669, Abcam) or Article Title: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity Article Snippet: Blocking for 1 h with Antibody Diluent (Dako) was followed by overnight incubation at 4 °C with the following primary antibodies: |